“Cepas nativas de Trichoderma para el control del moho gris (Botrytis cinerea Pers.) de arándano (Vaccinium corymbosum L.)”
No Thumbnail Available
Files
Date
2023-08-01
Journal Title
Journal ISSN
Volume Title
Publisher
Universidad Nacional de Cañete
Abstract
El objetivo de esta investigación fue determinar el efecto de las cepas nativas de Trichoderma para el control del moho gris (B. cinerea) de arándano (Vaccinium corymbosum L.) con un diseño completo al azar (DCA) con 19 tratamientos y 3 repeticiones por tratamiento, y para los bioensayos con un arreglo factorial 8 x 3 (Cepas x Dosis). Se evaluó la antibiosis con la extracción de metabolitos secundarios de las cepas nativas para evaluar la inhibición micelial de B. cinerea y micoparasitismo con el método de placa dual para evaluar la colonización del patógeno. Se seleccionaron siete cepas con capacidad antagónica para los bioensayos con dosis de metabolitos secundarios y suspensión de esporas donde se evaluó la incidencia y severidad del moho gris en flores y frutos de arándano. Todas las cepas nativas de Trichoderma colonizaron completamente al patógeno, considerándose como micoparásitos agresivos, por otro lado, la cepa HE-ArR7 tuvo una mayor actividad inhibitoria hasta un 42.74%; la aplicación de la dosis 3 de metabolitos secundarios mostró efectos significativos sobre la incidencia y severidad del moho gris en flores y frutos, donde las cepas HE-ArR1 (66.67%) y HE-ArR7 (17.33%) sobresalieron a nivel flores mientras que la cepa HE-ArR3 (50 % y 30.67% respectivamente) en frutos. Asimismo, la aplicación de suspensión de esporas de Trichoderma tuvo efectos inhibitorios sobre el moho gris destacando la cepa HE- ArT161 quien suprimió la incidencia en un 66.67% y 20% y la severidad en un 34% y 12 % en flores y frutos. Concluyendo así que el uso de cepas nativas de Trichoderma frente a B. cinerea estimula la inhibición y desarrollo del patógeno.
The objective of this research was to determine the effect of native Trichoderma strains for the control of blueberry (Vaccinium corymbosum L.) gray mold (B. cinerea) with a complete randomized design (DCA) with 19 treatments and 3 repetitions per treatment, and for the bioassays with a factorial arrangement 8 x 3 (Strains x Dose). Antibiosis was evaluated with the extraction of secondary metabolites from the native strains to evaluate the mycelial inhibition of B. cinerea and mycoparasitism with the dual plate method to evaluate the colonization of the pathogen. Seven strains with antagonistic capacity were selected for the bioassays with doses of secondary metabolites and spore suspension where the incidence and severity of gray mold on blueberry flowers and fruits was evaluated. All the native strains of Trichoderma completely colonized the pathogen, being considered as aggressive mycoparasites, on the other hand, the HE-ArR7 strain had a higher inhibitory activity up to 42.74%; The application of dose 3 of secondary metabolites showed significant effects on the incidence and severity of gray mold in flowers and fruits, where the HE-ArR1 (66.67%) and HE-ArR7 (17.33%) strains stood out at the flower level while the HE-ArR3 strain (50 % and 30.67% respectively) in fruits. Likewise, the application of suspension of Trichoderma spores had inhibitory effects on gray mold, highlighting the HE-ArT161 strain, which suppressed the incidence by 66.67% and 20% and the severity by 34% and 12% in flowers and fruits. Thus concluding that the use of native strains of Trichoderma against B. cinerea stimulates the inhibition and development of the pathogen.
The objective of this research was to determine the effect of native Trichoderma strains for the control of blueberry (Vaccinium corymbosum L.) gray mold (B. cinerea) with a complete randomized design (DCA) with 19 treatments and 3 repetitions per treatment, and for the bioassays with a factorial arrangement 8 x 3 (Strains x Dose). Antibiosis was evaluated with the extraction of secondary metabolites from the native strains to evaluate the mycelial inhibition of B. cinerea and mycoparasitism with the dual plate method to evaluate the colonization of the pathogen. Seven strains with antagonistic capacity were selected for the bioassays with doses of secondary metabolites and spore suspension where the incidence and severity of gray mold on blueberry flowers and fruits was evaluated. All the native strains of Trichoderma completely colonized the pathogen, being considered as aggressive mycoparasites, on the other hand, the HE-ArR7 strain had a higher inhibitory activity up to 42.74%; The application of dose 3 of secondary metabolites showed significant effects on the incidence and severity of gray mold in flowers and fruits, where the HE-ArR1 (66.67%) and HE-ArR7 (17.33%) strains stood out at the flower level while the HE-ArR3 strain (50 % and 30.67% respectively) in fruits. Likewise, the application of suspension of Trichoderma spores had inhibitory effects on gray mold, highlighting the HE-ArT161 strain, which suppressed the incidence by 66.67% and 20% and the severity by 34% and 12% in flowers and fruits. Thus concluding that the use of native strains of Trichoderma against B. cinerea stimulates the inhibition and development of the pathogen.
Description
Keywords
metabolitos secundarios, antagonismo
Citation
Arestegui Cantoral, Yasmin Carmen, “Cepas nativas de Trichoderma para el control del moho gris (Botrytis cinerea Pers.) de arándano (Vaccinium corymbosum L.)” Tesis para optar el grado de Ingeniero Agrónomo, Escuela de pregrado. Facultad de Agronomía. Universidad Nacional de Cañete. Cañete - Perú