Isolation, characterization and pathogenicity fungi associated with petri's disease
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Date
2023-03-08
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Universidad Nacional de Cañete
Abstract
Perú se ha convertido en el primer exportador de uva de mesa centrando su producción en las regiones de Piura e Ica. Sin embargo, la mayoría de los viñedos jóvenes (<5 años) en la región de Piura, vienen presentando síntomas de la enfermedad de Petri. Objetivo: Aislamiento, identificación y evaluación de patogenicidad de hongos asociados con la enfermedad de Petri. Metodología: Los aislados fueron obtenidos a partir de estrías necróticas en haces vasculares en plantas con síntomas de la enfermedad de Petri. La identificación se realizó con las claves propuestas por Mostert, mediante observaciones macro y microscópicas. Asimismo, se realizó las pruebas de patogenicidad midiendo el avance de la longitud de la estría necrótica LEN. Resultados: Por sus características culturales, microscópicas se identificaron a: Phaeoacremonium spp, P. krajdenii, P. inflatipes, P. parasiticum y Phaeomoniella chlamydospora. Todos los aislados seleccionados fueron patogénicos en las pruebas de patogenicidad 75 días después de la inoculación (DDI), siendo las especies de Phaeoacremoniumlas que presentaron mayor LEN comparadas con Pa. chlamydospora, lo cual demuestra su capacidad de colonización de haces vasculares y la degradación de la madera. Implicaciones: Los patógenos Phaeoacremonium spp, P. krajdenii, P. inflatipes, P. parasiticum y Phaeomoniella chlamydospora están relacionados con la enfermedad de Petri en la región Piura-Perú. Conclusión: Las especies de Phaeoacremonium mostraron mayor patogenicidad que Pa. chlamydospora 75 DDI. Así también, se identificó morfológicamente P. inflatipes, en la región Piura requiriendo la identificación molecular para su corroboración. Palabras clave: Enfermedad de petri; identificación morfológica; patogénico; manejo sostenible, Vitis vinífera L.
Peru has become the leading exporter of table grapes, focusing its production in the Piura and Ica regions. However, most of the young vineyards (<5 years) in the Piura region have been showing symptoms of Petri disease. Objective: Isolation, identification and evaluation of pathogenicity of fungi associated with Petri disease. Methodology: The isolates were obtained from necrotic streaks in vascular bundles in plants with symptoms of Petri disease. The identification was made with the keys proposed by Mostert, through macro and microscopic observations. Likewise, pathogenicity tests were performed by measuring the advancement of the length of the LEN necrotic streak. Results: Due to their cultural and microscopic characteristics, the following were identified: Phaeoacremonium spp, P. krajdenii, P. inflatipes, P. parasiticum and Phaeomoniella chlamydospora. All the selected isolates were pathogenic in the pathogenicity tests 75 days after inoculation (DDI), being the Phaeoacremonium species those that presented higher LEN compared to Pa. chlamydospora, which demonstrates its ability to colonize vascular bundles and the degradation of timber. Implications: The pathogens Phaeoacremonium spp, P. krajdenii, P. inflatipes, P. parasiticum and Phaeomoniella chlamydospora are related to Petri disease in the Piura-Peru region. Conclusion: Phaeoacremonium species showed higher pathogenicity than Pa. chlamydospora 75 DDI. Likewise, P. inflatipes was morphologically identified in the Piura region, requiring molecular identification for its corroboration. Keywords: Petri disease; morphological identification; pathogenic; sustainable management, Vitis vinifera L.
Peru has become the leading exporter of table grapes, focusing its production in the Piura and Ica regions. However, most of the young vineyards (<5 years) in the Piura region have been showing symptoms of Petri disease. Objective: Isolation, identification and evaluation of pathogenicity of fungi associated with Petri disease. Methodology: The isolates were obtained from necrotic streaks in vascular bundles in plants with symptoms of Petri disease. The identification was made with the keys proposed by Mostert, through macro and microscopic observations. Likewise, pathogenicity tests were performed by measuring the advancement of the length of the LEN necrotic streak. Results: Due to their cultural and microscopic characteristics, the following were identified: Phaeoacremonium spp, P. krajdenii, P. inflatipes, P. parasiticum and Phaeomoniella chlamydospora. All the selected isolates were pathogenic in the pathogenicity tests 75 days after inoculation (DDI), being the Phaeoacremonium species those that presented higher LEN compared to Pa. chlamydospora, which demonstrates its ability to colonize vascular bundles and the degradation of timber. Implications: The pathogens Phaeoacremonium spp, P. krajdenii, P. inflatipes, P. parasiticum and Phaeomoniella chlamydospora are related to Petri disease in the Piura-Peru region. Conclusion: Phaeoacremonium species showed higher pathogenicity than Pa. chlamydospora 75 DDI. Likewise, P. inflatipes was morphologically identified in the Piura region, requiring molecular identification for its corroboration. Keywords: Petri disease; morphological identification; pathogenic; sustainable management, Vitis vinifera L.
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Isolation, characterization and pathogenicity fungi associated with petri's disease, Articulo - Escuela Profesional de Agronomia